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KMID : 0376419850090040353
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Chonbuk University Medical Journal
1985 Volume.9 No. 4 p.353 ~ p.358
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Immune Reaction and Efficacy of Human Immunoglobulin on Group B Streptococcal Infection
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Abstract
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Two human intravenous immunoglobulin(IVIG) preparations modified by different methods (reduction and alkylation or pH 4 treatment) were evaluated for in vivo activity against a type 111 group B streptococcus(GBS) strain. the amount of IgG, IgA and IgM in both IVIG preparations were measured by radial immunodiffusion with goat anti-human IgG, IgA and IgM and the amount of IgG subclrss-~s were also measured by radial immunodiffusion with mouse monoclonal anti-human IgGI or IgG2(for IgGI, IgG2), and solid phase radioimmunossly with I125-rad,iolabelled mouse monoclonal anti-human IgG3 or IgG4 and filter paper discs saturated with purified pig polyclonal anti-human IgG3 or IgG4(for IgG3, IgG4).
Both IVIG preparations contained simialr amounts of IgG and IgA. IgM was not detectable in either, IVIG. IgG3 was not detectable in reduced-alkylated IVIG but 291.9mg/dl in pH 4treated IVIG. Otherwise, both IVIG preparations contained similar amounts of IgGi, IgG2 and IgG4.
In vivo, both preparations revealed protective against type In GBS, but pH 4-treated IVIG was significantly more protective against type 111 GBS. This was shown by lower magnitude of bacteremia, improved survival rate and lower PD50 in recipients of pH 4-treated IVIG. The mechanise for this apparent discrepancy in functional activities of IVIG is not clear, but it is conceivable that IgG3 may play an important role in protection against type Iff GBS infection, and changes on Fc-portion or chemical structure of IgG molecule may influence on its biological functions.
Futher studies will be needed to understand immune responses on GBS infection and why reduced-alkylated IVIG is less effective than pH 4-treated IVIG in vivo against GBS infection.
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